摘要
目的 研究盐酸小檗碱片中盐酸小檗碱的含量测定方法 ,并与中国药典方法的测定结果比较 ,评价两种方法的相关性。方法 用重铬酸钾比色法测定含量 ,以 42 1nm为测定波长 ,0 .2mg·mL-1的基准重铬酸钾溶液作对照液。结果 盐酸小檗碱在 4.0～ 2 8.0 μg·mL-1之间呈良好的线性关系 ,相关系数r=0 .9999,平均回收率为 10 0 .0 5 % (n =5 ) ,RSD为 0 .43%。比较本法和中国药典方法的测定结果 ,相关性显著。结论 本法简便、准确、重现性好 ,可用于盐酸小檗碱的含量测定。
OBJECTIVE: To develop an assay for the determination of berberine hydrochloride in berberine hydrochloride tablets, and to compare the results measured by colormetric method with the assay described in Chinese Pharmacopoeia 1995 for assessing the correlation of the two methods. METHODS: Potassium dichromate colormetric method was selected to determine the content, and the 0.2 mg·mL-1Potassium dichromate was used as the reference solution. The absorbency was measured at 421 nm. RESULTS: The calibration curve for berberine hydrochloride was linear within the concentration range of 4.0∼28.0 μg·mL-1 (r = 0.9999). The average recovery was 100.05 % (n = 5), and the RSD was 0.43 %. The analytical results for berberine hydrochloride between the two methods was found to be correlated. CONCLUSION: The method was proved to be simple, precise and reproduciable. It can be used for the quantitative determination of berberine hydrochloride. It is especially practicable for rapid analysis in production.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2001年第4期274-275,共2页
Chinese Pharmaceutical Journal
关键词
重铬酸钾比色法
盐酸小檗碱
含量
抗菌药
Assays
Calibration
Colorimetric analysis
Potassium compounds
