摘要
目的 :建立重感灵片中安乃近及马来酸氯苯那敏的含量测定方法。方法 :样品经Sep PakC18微柱处理后用离子对高效液相色谱法测定。色谱条件 :HypersilBDS C18填充柱 (2 5 0mm× 4.6mm ,i.d .5 μm)为分析柱 ;甲醇 庚烷磺酸钠醋酸混合液 (6 0 0∶40 0 )为流动相 ;检测波长为 2 6 4nm ;流速为 :1mL/min。结果 :测得安乃近的平均回收率为 :99.6 % ,RSD =2 .1% (n =6 ) ,马来酸氯苯那敏的平均回收率为 :98.0 % ,RSD =1.5 % (n =6 )。结论 :用Sep PakC18微柱固相萃取法代替传统的回流、提取、浓缩的样品预处理 ,选择庚烷磺酸钠离子对试剂在酸性条件下 ,用高效液相色谱法以同一流动相 。
Objective: To establish a method for the determination of metamizole sodium and chlorphenamine malete in zhongganling Tablets. Methods: The sample was determined by ion pair HPLC after it was purified on Sep Pak C 18 microcolumn. The chromatographic conditions included: Hypersil DBS C 18 chromatographic column (250mm×4.6mm, i.d.5μm) as an anlaytical column, methanol mixed solution of sodium heptanesulfonate and glacial acetic acid (600∶400) as a mobile phase, the detection wavelength at 264nm and 1.0mL·min -1 of flow rate. Results: The average recoveries of metamizole sodium and chlorphenamine maleate were 99.6% (RSD was 2.1% and n was 6) and 98.0% (RSD was 1.5% and n was 6), respectively. Conclusion: Metamizole sodium and chlorphenamine maleate can be determined respectively by HPLC with the same mobile phase when Sep Pak C 18 microcolumn solid phase extraction method is used to substitute for the traditional sample pretreatment methods refluxing, extracting and concentrating, and sodium heptanesulfonate ion pair reagent in acid condition is selected.
出处
《中成药》
CAS
CSCD
北大核心
2001年第4期246-249,共4页
Chinese Traditional Patent Medicine
