摘要
目的 建立用超滤法快速分离六味地黄汤中免疫活性多糖的工艺。方法 利用两支不同孔径中空纤维超滤柱截留活性多糖部位 ;采用凝胶柱层析结合硫酸 -苯酚显色法 ,测定多糖的分子量 ;利用酸水解和TLC测定多糖的主要糖组成。结果 得到相对分子量 3万左右的多糖组分。结论 超滤法分离多糖是一种有效的方法。工艺简单 。
Aim To establish a rapid method of isolating bio-active polysaccharides from LWDH by superfiltration.Methods The bio-active fractions of LWDH polysaccharides were isolated with two superfiltration-columns of different pore-size and with sulphuric acid-phenol to test collected fractions.The molecular weight of the polysaccharides were measured by gel filtration chromatography.The sugar composition of the polysaccharides were determined by TLC after acidic hydrolysis.Results Obtained a polysaccharide fraction of molecular weight about 30 000 from LWDH.Conclusion Superfiltration is proved to be a simple,time-saving method for the isolation of herbal polysaccharides.
出处
《解放军药学学报》
CAS
2001年第2期69-70,共2页
Pharmaceutical Journal of Chinese People's Liberation Army
基金
国家自然科学基金重点项目资助课题!No .39730 480
关键词
六味地黄汤
超滤法
多糖
凝胶柱层析
中药
LWDH decoction
Superfiltration
Polysaccharides
Gel filtration chromatography
