摘要
目的 :探索人胃癌和胃粘膜上皮原代细胞在体外不含甲硫氨酸 (Met)而含同型半胱氨酸 (Hcy)培养基 (Met-Hcy+)中能否正常生长及是否存在Met依赖性。 方法 : 将新鲜人胃癌及胃粘膜组织制成单细胞悬液 ,分别置于Met-Hcy+和Met+Hcy-培养基中培养 ,利用细胞计数和流式细胞分选仪检测各组细胞增殖状态。 结果 :人胃癌原代细胞在Met-Hcy+培养基中生长受抑 ,表现为细胞总数减少 ,G0 、G1期细胞比例下降 ,S期细胞比例升高。胃粘膜细胞在Met-Hcy+培养基中生长正常 ,细胞总数及细胞周期比例未受影响。 结论 :人胃癌原代细胞在体外培养中表现出Met依赖性 ,而胃粘膜细胞则未见类似现象。
Objectives:To study the methionine dependence (Met-dependence) of human primary gastric cancer cells in vitro when Met in the culture medicine was replaced by its precussor homocysteine(Hcy). Methods:Fresh and sterile gaotric cancer samples and normal gastric mucosa were managed into single-cell suspension and were cultured in the Met -Hcy + and Met\++ Hcy\+- medium separately.proliferation of these cells in the two different culture media was examined by cell counter and flow cytometry (FCM). Results:In the Met\++Hcy\+- medium,the growth of primary gastric cancer cells was inhibited,manifesting that the percentage of G 0G 1 tumor cells decreased and that of the S phase increased signiflcantly.However,normal gastric mucosal cells grew well and their cell cycles had no change. Conclusions:Human primary gastric cancer cells are Met dependent in vitro whereas normal gastric mucosal cells have no similar results.
出处
《肠外与肠内营养》
CAS
2001年第2期76-79,共4页
Parenteral & Enteral Nutrition
基金
国家卫生部科研基金!资助项目 (No96 -2 -2 96 )
