摘要
以X-XOD体系产生O_2、Luminol作为发光增强剂,用化学发光法测定了12种黄酮单体清除O_2反应的速率常数(k_3),其k_3值在10^(-5)～10^(-6)(mol/L)^(-1)sec^(-1)之间。以k_3值的大小作为比较指标,对黄酮体的结构与清O_2活力之问的关系进行了探讨。同时又用化学发光法测定了这些黄酮试样在1mol/L浓度时对V_C-H_2O_2-Cu^(2+)-酵母多糖体系产生的·OH的抑制,显示出黄酮类化合物在清除O_2和·OH时的活性部位和作用机制并不完全相同,认为4’-OH和△^(2(3))双键是清O_2的主要活性部位,而B环邻二羟基(3’,4’-di-OH)则是清除·OH的关键功能团。
The reaction rate constants (k3) of scavenging O2 of 12 species of flavonoids were determined by Chemiluminescene(CL) ,taking the X-XOD system to produce O2 and luminol as a promoter,which located in the range of 10-6-10-6mol/L-1sec-1. The relationship between the structure of flavonoids and their scavenging activity on O2 was analyzed taking k3 as comparing indexes. At the same time,the inhibition rates of these flavonoids at 1mmol/L to · OH produced by Vc-H2O2-Cu2+-yeast-polysaccarides were also measured by CL. Results show that the active sites and reaction mechanism of flavonoids to scavenge O2 and · OH were not completely same,and 4'-OH and Δ2(3) double-bond seem to be the key sites to O2 and 3' ,4'-di-OH was main active group to · OH,respectively.
出处
《天然产物研究与开发》
CAS
CSCD
1998年第4期26-33,共8页
Natural Product Research and Development
基金
国家八.五攻关项目经费资助
关键词
黄酮类化合物
活性氧自由基
清除能力
构效关系
flavonoids,active oxygen radicals,scavenging capacity,relationship between structure andactivity
