摘要
目的 诱导能模拟人类Alzheimer病 (AD)的大鼠病理模型 ,用于该病的病理机制和治疗药物的研究。方法 采用双侧海马内一次性注射 β 淀粉样多肽 2 5～ 35片段(Aβ2 5～ 35)诱导大鼠AD模型 ;行为测试采用跳台法、穿梭法和Morris水迷宫法 ;病理检测采用HE染色、刚果红染色和银染。结果 跳台法、穿梭法和Morris水迷宫法试验结果提示海马内注射Aβ2 5～ 35可引起大鼠主动和被动回避性反射及空间分辨力降低 ;病理检查发现皮质和海马神经元数量减少、退变 ,皮质下血管淀粉样变及脑内出现纤维蛋白丝状物。结论 海马内注射Aβ2 5～
AIM To determine whether microinjection of β amyloid peptide fragment 25～35 (Aβ25～35 ) into rat hippocampus induces the learning and memory dysfunction.METHODS Microinjection of Aβ 25～35 into hippocampus induced the rat learning and memory dysfunction. Step down test, Shuttle box test, and Morris water maze test were used to evaluate rat learning and memory function;The sections were stained with haematoxylin eosin (HE),the amyloid deposits were detected using Congo Red and silver.RESULTS The latency of Aβ 25～35 treated rats shortened,the stimulating time prolonged,and the numbers of errors increased in the step down test;Shuttle box test showed that Aβ 25～35 caused the latency and numbers of active escape reduction and the numbers of passive avoidance increase; Also in morris water maze, Aβ25 35 induced an impairment in rat spatial resolution; In Aβ 25～35 treated group, a significant decrease in the numbers of neurons in cortex and hippocampus,a massive glial reaction and neurophilic phenomenon were detected by HE staining; the positive vascular amyloidosis by Congo red and fibrils by Ag staining were also observed.CONCLUSION Microinjection of Aβ 25～35 into rat hippocampus may induce the learning and memory dysfunction and pathological changes which were similar to that found in Alzheimers disease (AD) brain.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2001年第1期26-29,共4页
Chinese Pharmacological Bulletin
基金
安徽省教育厅科技基金资助!No 98JL0 6 6
