摘要
目的 研究四次甲基二砜四胺 (TET)对急性中毒动物的致惊作用及机制。方法 通过急性毒性实验 ,测定小鼠CD97;使用氨基酸自动分析仪测定小鼠脑内游离氨基酸含量 ;用放射自显影实验和图像分析系统测定氚片中不同脑区的光密度值。结果 TET急性中毒小鼠的CD97为 0 384(0 337～ 0 431)mg kg ;TET中毒小鼠脑内游离氨基酸γ 氨基丁酸 (GABA)含量显著增加 ,谷氨酸含量显著降低 ;放射自显影实验表明TET显著降低氚片中不同脑区的光密度。结论 TET具有强烈的中枢兴奋作用 ,中毒动物因强直性惊厥的反复发作致呼吸衰竭而死亡 ,推测致惊机理与直接抑制脑内GABA和受体的结合有关。
Objective To study the convulsive effects and mechanisms of tetramethylenedisulphotetramine.Methods The 97% of convulsive dose(CD 97 ) was determined;the contents of free amino acid were measured with amino acid autoanalyzer in mice brain;[ 3H] γ\|aminobutyric acid(GABA)binding sites in the mice brain were observed by autoradiography (ARG) and the density was determined with the image analysis system.Results CD 97 was 0\^384 mg/kg[(0\^337～0\^431)mg/kg] in acutely poisoned mice; the contents of free GABA increased and glutamic acid decreased significantly and other amino acid was no significant differences in mice brain;The lowered density of different brain regions was observed in hyperfilm.Conclusion TET has strong stimulating effect on the central nervous system and the reason of death is respiratory failure caused by continuous seizure of tetanic convulsion in acutely poisoned animals;the convulsive mechanisms may be related to the direct inhibition of [ 3H] GABA binding with its receptor.
出处
《卫生毒理学杂志》
CSCD
北大核心
2001年第1期5-7,共3页
Journal of Health Toxicology
基金
浙江省教委资助!( 990 45 4)
关键词
四次甲基二砜四胺
惊厥
毒理
Tetramethylenedisulphotetramine
Convulsion
Mechanism
