摘要
目的 研究弓形虫抗原对免疫宿主的保护性。 方法 用 SDS- PAGE大胶板电泳对弓形虫抗原进行分离 ,选择 4组份特异抗原带 (P2 2～ 2 6、P30、P35、P6 7)洗脱获得抗原后 ,分别与福氏完全佐剂混匀免疫小鼠 (每组 10只 ) ,发现P30诱导的小鼠抗弓形虫 Ig G抗体出现最早 ,滴度最高 ,其次为 P35。以毒力减弱的 RH株弓形虫对免疫后的各组小鼠进行攻击。 结果 对照组、P2 2～ 2 6、P30、P35和 P6 7组小鼠的平均存活时间分别为 7.1、7.8、8.3、8.0和 7.3d。 结论P30对小鼠具有较强的保护力 (P<0 .0 5 ) ,次为 P35。
Objective To study the protection of Toxoplasma gondii antigens. Methods Antigens of T. gondii were segregated with SDS PAGE electrophoresis. Four fractional antigens of T. gondii (P22-26, P30, P35 and P67) were eluted from the gels after electrophoresis and then mixed with Freunds complete adjuvant separately. Five groups of Kunming mouse (10 mice each) were selected and immunized respectively with the four mixers and the adjuvant. Results\ P30 could induce the earliest and highest IgG response and P35 followed. After they were challenged with toxicity reduced T. gondii (RH), the mean survival times of the immunized mice in the groups of control, P22-26, P30, P35 and P67 were 7.1, 7.8, 8.3, 8.0 and 7.3 days respectively. Conclusion\ P30 had the strongest protective action against T. gondii ( P <0.05) and P35 took the second place. Both P30 and P35 were likely to be candidate antigens for vaccine.
出处
《中国寄生虫病防治杂志》
CSCD
2001年第1期5-7,共3页
Chinese Journal of Parasitic Disease Control
关键词
弓形虫
抗原
保护性免疫力
Toxoplasma gondii
antigen
protection immunity
