摘要
目的 研究日本血吸虫 (中国大陆株 ) 2 2 .6 k Da有效抗原表位对小鼠的免疫保护性 ,预测其在抗血吸虫感染中的作用。方法 用纯化的抗 Sj2 2 .6 k Da的多克隆抗体 Ig G对噬菌体 12肽库进行 5轮免疫学筛选 ,挑取克隆 ,经 Western- blot免疫识别、动物初筛及序列分析后 ,将获得的阳性克隆免疫 BABL/ c小鼠。结果 共获得 4个有效抗原表位 ,各免疫组和对照组相比 ,4种表位混合免疫组小鼠 ,获得了 39.5 % (P<0 .0 5 )的减虫率及 5 7.1% (P<0 .0 1)肝总减卵率 ;4种表位分别免疫4组小鼠 ,各组分别获得了 2 7.5 % (P<0 .0 5 )、5 .4% (P<0 .0 5 )、2 2 .8% (P<0 .0 5 )、11.6 % (P<0 .0 5 )的减虫率及 41.4% (P<0 .0 1)、2 9.7% (P<0 .0 5 )、32 .2 % (P<0 .0 5 )、30 .9% (P<0 .0 5 )肝总减卵率。结论 所获得 4种有效抗原表位均可诱导宿主抗血吸虫感染的部分保护力 。
Objective To evaluate the immunoprotective effect of the epitopes of 22 6 kDa antigen of Schistosoma japonicum Methods A 12 mer phage was used to display library to screen the epitopes of Sj22 6 kDa with the purified polyclone IgG antibody against the antigen. Five rounds of biopanning were carried out and sixteen clones from the fifth round biopanning were randomly selected, identified by Western blot and sequenced. The mice were immunized with the obtained positive clones. Results Four epitopes of Sj22 6 kDa were obtained. In BABL/c, the mixture of four epitopes could induce 39 5%(P<0 05) worm reduction and 57 1%(P<0 01) total liver egg reduction. Four different clones could respectively induce 27 5%(P<0 05), 5 4%(P<0 05), 22 8%(P0 05), 11 6%(P<0 05) worm reduction and 41 4%(P<0 01), 29 7%(P<0 05), 32 2%(P<0 05), 30 9%([WT5”BX]P[WT5”BZ]<0 05) total liver egg reduction. Conclusion The four epitopes of Sj22 6 kDa antigen are partially effective against S. japonicum.]
出处
《中国血吸虫病防治杂志》
CAS
CSCD
2001年第2期86-89,共4页
Chinese Journal of Schistosomiasis Control
基金
总理预备金血防专项基金! (No.94-y-2 3)资助
