摘要
目的 :研究脑缺血再灌后p5 3基因表达变化。方法 :采用沙鼠短暂前脑缺血再灌损伤模型 ,应用Northernblot检测脑缺血再灌后不同时期额叶p5 3mRNA表达。结果 :沙鼠脑缺血 6min ,在再灌后 6hp5 3mRNA出现表达增加 ,持续至第 3d(P <0 .0 5 )。结论 :沙鼠短暂脑缺血后 ,促凋亡基因p5 3存在持续高表达 ,其结果可能是启动细胞凋亡 ,导致缺血神经细胞损伤死亡。
Objective To study the expression of p53 gene after transient ischemia in the gerbil. Methods Northern blot was used to detect the expression of the p53 mRNA in the forebrain during different periods after post ischemic reperfusion in the gerbil. Results The expression of p53 mRNA increased in the forebrain during the 6th hour to the 3rd day of reperfusion following 6 min of ischemia (P<0.05). Conclusion The high level expression of p53 might induce neuronal apoptosis after ischemia.
出处
《湖南医科大学学报》
CSCD
北大核心
2001年第2期121-122,共2页
Bulletin of Hunan Medical University
