摘要
目的 探讨Ｇａｌａｒｄｉｎ对角膜基质细胞胶原降解的抑制作用。方法角膜基质细胞三维胶原凝胶表面分别添加纤维蛋白溶酶原、白细胞介素－１α（ＩＬ－１α）及不同质量浓度的 Ｇａｌａｒｄｉｎ的 ＭＥＭ培养液培养 ２４ ｈ，测定培养上清液中羟脯氨酸的量做为胶原降解活性单位。向时检测 Ｇａｌａｒｄｉｎ及 ＩＬ－１α对角膜基质细胞胶原降解的作用。结果在存在纤维蛋白溶酶原的情况下，随着 ＩＬ－１α质量浓度的升高，以剂量关系增加角膜基质细胞胶原降解量。 Ｇａｌａｒｄｉｎ以剂量关系抑制 ＩＬ－１α诱导的角膜基质细胞胶原降解量。结论Ｇａｌａｒｄｉｎ具有抑制 ＩＬ－１α诱导的角膜基质细胞胶原降解的作用。
ObjectiveTo study the inhibitive effect of Galardin on keratocyte collagen degradation.Methods Keratocyte collagen gels were incubated with plasminogen,interleukin-1α(IL-1α)and different concentration Galardin MEM culture medium for 24 hours separately.The ultrafiltrates were measured then hydrolyzed,and the amount of hydroxyproline were measured spectrophotometrically.The effect of a synthetic matrix metallaproteinase(MMP)inhibitor,Galardin,and interleukin-1α (IL-1α)on collagen degradation were also examined.ResultsIn the presence of plasminogen,IL-1α increased keratocyte collagen degradation in a dosedependent fashion.Galardin suppressed IL-1α-induced collagen degradation more strikingly in a dose dependent fashion.ConclusionGalardin inhibites keratocyte collagen degradation induced by IL-1α.
出处
《眼科研究》
CSCD
北大核心
2001年第2期123-124,共2页
Chinese Ophthalmic Research
