摘要
目的 :探讨转化生长因子 β1(TGF β1)在肺纤维化中的作用。方法 :采用博莱霉素 (BLM)致肺纤维化大鼠模型 ,分离纯化肺泡巨噬细胞 (alveolarmacrophage ,AM) ,用RT PCR及图像分析方法 ,对不同时期AM以及肺组织释放的TGF β1进行动态观察。结果 :BLM组AM和肺组织中TGF β1mRNA第 3天即明显增加 (P <0 .0 5和P>0 .0 5 ) ,第 7天达到高峰 (P<0 .0 1) ,第 142 8天逐渐下降 ,但仍高于对照组 (P>0 .0 5 )。结论 :TGF β1mRNA在正常大鼠及肺纤维化大鼠中均有表达 ,后者表达明显高于前者 ;肺纤维化大鼠肺内TGF β1mRNA可能主要来源于AM。
Objective: Our aim was to evaluate the role of TGF β 1 mRNA in pulmonary fibrosis. Methods: The present study was taken to investigate the expressions of TGF β 1 mRNA in alveolar macrophage(AM) and lung tissues in different stages of bleomycin induced pulmonary fibrosis in rats through reverse transcript polymerase chain reaction. Results: In bleomycin treated rats, the expressions of TGF β 1 mRNA was remarkably increased in AM from 3rd day (P< 0.05) , and reached the peak on 7th day (P<0.01) , there was also a continuing high level from 14th day to 28th day (P>0.05). It showed similar pattern with that in lung tissues. Conclusion: There were TGF β 1 mRNA expression in both normal rats and pulmonary fibrosis rats. AMs seemed to be the main source of TGF β 1 mRNA in lung tissues, especially at stage of alveolitis .
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2001年第2期94-95,100,共3页
Journal of China Medical University
关键词
转化生长因子Β1
逆转录聚合酶链反应
肺纤维化
博莱霉素基因表达
transforming growth factor beta 1
alveolar macrophage
reverse transcript polymerase chain reaction
pulmonary fibrosis
