摘要
最近的研究表明，果蝇与脊椎动物及人的心脏早期发育具有极为相似的基因控制机理，果蝇已成为研究人体心脏早期发育基因控制的理想模式动物。利用化学诱变剂甲磷酸乙酯大规模地诱变影响果蝇心脏发育的基因，利用心脏特异性抗体染色进行筛选，获得了１１２个有心脏突变表型的致死系，其中３２个致死系的心脏畸变表型有别于目前已知心脏发育基因的突变表型。细胞遗传学定位研究表明在多线染色体的１３个带纹区内的某些隐性致死突变基因是目前未知的，其功能可能与心脏发育有关的基因。
Rescent studies suggest that the basic molecular control mechanisms of early heart development are remarkably conserved in Drosophila, vertebrate and human being. Drosophila can be used as a prototype to explore the genetic basis of cardiogenesis in human being. Here, mutations of genes affecting heart development of Drosophila are produced by chemical mutagen methanesulfonicaeid ethyl. With staining of antibody expressed in heed precussor cells of Drosophila, 112 lethal lines were observed to show mutant phenotypes in pericardial cells. of them, 32 lines differ in their mutant phenotypes from those of known genes. Analysis of cytogenetic mapping shows that they are located in 13 chromosomal regions without known heart-related genes, which implies that these loci contain genes probably involved in the beau development.
基金
国家自然科学基金资助!(批准号:39770382)
关键词
果蝇
心脏发育
基因突变
抗体筛选
Drosophila
heart development gene mutation
antibody screening
