摘要
DNA of human cytomegalovirus (HCMV) contains three transforming fragments, which have been mapped in the long unique region of the viral genome(see Fig.1). A minimal region of 558 base pairs(bp) (pCM4127) was localized in the XbaI-Hindlll fragment of HCMV strain AD169 (mapunit 0.123—0.140)and designated morphological transforming region Ⅰ (mtrⅠ). MtrⅠwas reported to cause one-step focal transformation of primary
出处
《中国病毒学》
CAS
CSCD
1991年第4期281-290,共10页
Virologica Sinica
关键词
人巨细胞病毒
转化活性
DNA序列分析
Southern印迹
启动子
氯霉素乙烯转移酶
Human Cytomegaloviruses Transformation Activity DNA Sequenee Analysis Southern Blot Hybridization S_1 Nuclease Analysis NIH 3T3 Cells Promoter Activity Chloramphenicol Acetyltransferase Assay
