摘要
目的 探讨宫颈上皮癌变过程中的细胞生物学特征及其变化规律 ,为临床诊治提供依据。方法 收集 12 5份宫颈组织行病理检查 ,按病变程度不同分为 ,正常宫颈上皮 11份、宫颈上皮内瘤变Ⅰ级 (CINⅠ ) 2 2份、宫颈上皮内瘤变Ⅱ级 (CINⅡ ) 17份、宫颈上皮内瘤变Ⅲ级 (CINⅢ ) 13份和宫颈鳞癌 5 5份 (其中高、中、低分化宫颈鳞癌分别为 13、19、2 3份 ) ,以及宫颈腺癌 7份。组织切片分别行福根 (Feulgen)染色 ,并以计算机检测其DNA平均倍体值 (DMP)、DNA指数 (DI)、核面积 ,打印DNA倍体直方图。结果 (1)随病变程度增高 ,DMP、DI和核面积测定值均依次逐渐增高 ,正常宫颈上皮与CINⅠ、CINⅡ 3者间比较 ,除正常宫颈上皮与CINⅡ的DI和核面积、CINⅠ与CINⅡ的核面积比较 ,差异有显著性 (P <0 0 5 )外 ,其余差异均无显著性 (P >0 0 5 ) ;而宫颈癌与CINⅠ、CINⅡ比较 ,差异均有极显著性 (P <0 0 1) ;高、中、低分化宫颈鳞癌 3者间比较 ,差异也均无显著性。 (2 )宫颈不同病变的DNA倍体直方图各有特点 ,超 5倍体 (5C)细胞在CINⅢ病变组织中开始增多 ,5C、7C和 9C细胞在鳞癌病变组织中显著增加。结论 宫颈上皮癌变是一个渐进的、由量变到质变的过程。
Objective The aim of this research was to study the biological and clinical features of cervical cancer and precursor lesions Methods Nuclear DNA was analyzed by image cytometry (ICM) in 125 embedded tissue 5 μm sections stained with Feulgen stain Samples included normal cervical squamous epithelium ( n =11), cervical intraepithelial neoplasiaⅠ (CINⅠ) ( n =22), CINⅡ ( n =17) and CINⅢ ( n =13), cervical neoplasm ( n =62) Results The mean DNA content, nuclear area increased progressively from normal cervical epithelium, CINⅠ , CINⅡ , CINⅢ to invasive squamous carcinoma Statistical analysis revealed significant difference ( P <0 05, <0 01) Histograms of cellular DNA ploidy distribution showed different pattern, where 9 components (9C) cells were observed in cervical cancer and CINⅢ No differences in DNA mean ploidy (DMP), DNA index (DI) and nuclear area were observed between cervical neoplasm Conclusions It is increased progressively from normal cervical epithelium to cancer, in which CINⅢ is turning point
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2001年第5期267-270,共4页
Chinese Journal of Obstetrics and Gynecology
