摘要
在碱性条件下,将3,5-二硝基水杨酸与乳糖共同加热后,乳糖可将前者还原成棕红色的氨基化合物,在一定的浓度范围内,棕红色物质的颜色的深浅与乳糖含量成正比。用双波长法扣除显色液中背景的吸收,可消除蛋白质等杂质的干扰,省去分离蛋白质这一步骤,使该法具有简便,快速等特点。
Lactose was heated together with 3,5-dinitrosalicylic acid underbasic condition and the latter was reduced to a red-brown amino compound bylactose.The colourity is postitively correlative to the lactose content in cert-ain regions.Dual-wavelength spectrophotometry was used to test the colourand correct for background absorbance in this experiment.This is an easy andfast method since it drops out the protein separation process.
关键词
牛乳
乳糖
双波长法
分光光度法
lactose
spectrophotometry/cow milk
3,5-dinitrosalicylic acid
dual wavelength spectrophotometry
