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南荻遗传转化系统的建立及转基因植株的获得 被引量:21

Establishment of Genetic Transformation System for Miscanthus Sacchariflorus and Obtained of Its Transgenic Plant
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摘要 建立了南荻愈伤组织高频诱导与再生系统及其转化愈伤组织的筛选系统 ,并通过本研究所建立的基因枪转化系统 。 The initiation and regeneration system for embryogenic callus of Miscanthus sacchariflorus were established at very high frequency. Potato proteinase inhibitor II ( pin II) gene were introduced into the callus of Miscanthus sacchariflorus and some transgenic plants were obtained by the particle bombardment transformation system. PCR and Southern analysis showed that target genes were integrated into the genome of these transgenic plants.
出处 《高技术通讯》 EI CAS CSCD 2001年第4期20-24,共5页 Chinese High Technology Letters
关键词 南荻 基因枪轰击 马铃薯 蛋白酶抑制基因 愈伤组织 转基因植株 Miscanthus sacchariflorus, Biolistic bombardment, Potato proteinase inhibitor gene, Callus
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