摘要
目的 :体外定向诱导成人骨髓间质干细胞 (MSC)分化为神经元样细胞。方法 :采用Ficoll -Paque液 (1 0 77× 10 3 g/L)离心分离成人MSC ,体外扩增 ,分别采用含巯基乙醇和硫代甘油等试剂的无血清DMEM诱导MSC分化为神经元。免疫组化鉴定神经元烯醇化酶 (NSE)、神经丝蛋白 (NF)、胶质纤维酸性蛋白 (GFAP)的表达。结果 :成人骨髓间质干细胞在体外扩增 5代可获 5× 10 7个细胞。加入巯基乙醇等或硫代甘油等诱导剂诱导后 ,MSC胞体收缩 ,突起伸出 ;免疫组化显示诱导出的神经元样细胞NSE、NF表达阳性 ,GFAP阴性。结论 :成人骨髓间质干细胞在体外可以分化为神经元样细胞。
AIM: To investigate the differentiation from human mesenchymal stem cells(hMSC) into neuron-like cells.METHODS: hMSC were separated from rib marrow with Ficoll-Paque reagent and expanded in culture medium. hMSC were induced to differentiate with DMEM/monothioglycerol or DMEM/β-mercaptoethanol, respectively. Neuron-specific enolase(NSE), neurofilament(NF), and glial fibrillary acidic protein(GFAP) were detected by immunohistochemistry. RESULTS: hMSC were expanded as undifferentiated cells in culture for more than 5 passages. When treated with monothioglycerol or β-mercaptoethanol for 5 hours, hMSC exhibited neuronal phenotype. The expression of NSE and NF in the neuron-like cells was positive, but the glial astrocyte marker GFAP didn't express. CONCLUSION: hMSC can be induced to differentiate into neurous.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2001年第5期385-387,I001,共4页
Chinese Journal of Pathophysiology
基金
国家重点基础研究发展规划项目! (973)资助(No .G19990 5 430 0 )
