摘要
用正交试验探讨了从马面■卵巢中提取磷脂的方法，得到最佳工艺条件：提取剂用量为样品重 14倍 ,提取剂中氯仿与甲醇体积比 2∶ 1,抽提时间 4 h， 洗涤分层时加滤液体积一半的 1％ NaCl溶液 ,其中前三个因素对实验结果有显著影响。
： The method of phospholipids extraction from Navodon septentrionalos(Gü nther) ovary was discussed by orthogonal trials .The results indicated that the optimum condition of extraction is as follows: extractant(ml):sample(g)=14:1, chloroform(ml):methanol(ml)=2:1, four hours'extraction, 1％ NaCl(ml):extratum(ml)=1:2.In addition， the results is affected apparently by the three factors except the volume of 1％ NaCl.
出处
《浙江海洋学院学报（自然科学版）》
CAS
2001年第1期35-37,共3页
Journal of Zhejiang Ocean University(Natural Science Edition)
