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Studies on Pattern of Producing Transgenic Fish II. Several Elements Related to the Establishment of Transgenic Fish

转基因鱼构建模式的研究II.构建转基因鱼的几个影响因素(英文)
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摘要 A recombinant plasmid (pcMTsGH) containing the common carp methlothionein promoter (cMT) and salmon growth hormone gene (sGH) was microinjected into common carp fertilized eggs and embryos to optimize the conditions for producing transgenic fish. Several key elements were found to have significant effects on the integration of exogenous gene. These elements include forms and doses of pcMTsGH gene microinjected, stages of embryo at which microinjection conducted and pretreatment of fertilized eggs for microinjection. The results show that, in producing transgenic fish, (1) the best time for microinjection is late onecell stage, (2) the most appropriate dose of pcMTsGH gene for microinjection is around 106~107 copies; (3) the hatching rate rises up when the gene is microinjected into the eggs whose chorions are removed, but the integration rate has no significant changes before and after the removal of egg chorions. StudiesonPaternofProducingTransgenicFishI.SeveralElementsRelatedtotheEstablishmentofTransgenicFishLIUChunqiao,ZHANGYongzhon...
出处 《Developmental and Reproductive Biology》 1997年第2期21-27,共7页 发育与生殖生物学报(英文版)
关键词 transgenic fish integration rate hatching rate 转基因鱼 构建模式 cMT-sGH融合基因
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