摘要
本文报道用反相高效液相色谱法测定黄连及含黄连的中成药中盐酸小檗硷的含量。实验结果表明:以0.4mol/L硝酸铵-甲醇(52:48)作为流动相可得到满意的分离效果。以1％H_2SO_4水溶液超声振荡2小时提取样品被测组分,方法简便易行。
This paper reports a re-versed phase high performance liquidchromatography method to determine ber-berine hydrochloride in Coptis chinensisFranch. and in the traditional Chinesemedicine containing Coptis chinensisphase of 0.4mol/L ammonium nitrate-methanol(52:48) can give a satisfactoryseparation. The extraction of samplewith1%H_2 SO_4 solution using the ultra-sonic vibration method is simple andeasy to operate.
出处
《华西医科大学学报》
CSCD
1991年第1期90-92,共3页
Journal of West China University of Medical Sciences
关键词
盐酸小檗硷
黄连
中成药
色谱法
Berberine hydrochlonide
Coptis chinensis Franch
Traditional
Chinese medicine
