摘要
4种桐乡产杭白菊及黄菊和野菊，分别用乙醇－水体系进行热回流提取，用比色法测定醇提物中总酚和总黄酮含量，并用化学发光法检测其清除活性氧自由基的能力。结果表明不同品种间含量存在较大差异，总酚和总黄酮含量（以菊花干基计）最高的是异种大白菊，分别为 9.76％和 4.46％；最低的是黄山种野菊，分别为 3.21％和 1.99％。不同菊花均显示了良好的清除活性氧自由基的活性。清· OH和 O_2·－的 IC 50分别在 0.54～ 1.98mg/ml和 24.2～ 54.5μ g/ml之间。其中清 O_2·－活性最强是大洋菊，最弱是软梗小洋菊；而清· OH能力最强为早小洋菊，最弱为异种大白菊。结果表明，菊花中酚类物质的含量高低与抗自由基活性强弱无明显的对应关系。
Six species chrysanthemum samples,including 4 Hangbaiju,1 Huangju and 1 Yeju,were collected from the famous“ Hangbaiju Kingdom of China',Tongxiang city,Zhejiang province.Fresh flower was dried by microwave oven assisted by hot air circulation,and extracted individually by thermal reflux with 70％ ethanol－ water solution.The contents of total phenols (TP) and total flavonoids (TF) of these extracts were measured by spectrophotometric analysis.Obvious differences were found in these chrysanthemums:Yizhongdabaiju had the highest contents at 9.76％ TP and 4.46％ TF (in dry base of flower),and Yeju was the lowest one at 3.21％ TPand 1.99％ TF.Chemiluminescence method demonstrated that all of samples had good scavenging capacity on reactive oxygen free radicals.The IC 50 ranged in 0.54～ 1.98mg/ml to· OH and 24.2～ 54.5μ g/ml to O_2·－ ,respectively.Among these chrysanthemums,Dayangju had the strongest scavenging activity to O_2·－ ,and Ruangengxiaoyangju was the weakest one;similarly,Zaoxiaoyangju had the strongest scavenging activity to· OH and Yizhongdabaiju was the weakest one.This research showed no obvious correlativity between the phenolic compound content of chrysanthemums and their anti－ free radical capacity.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2001年第4期84-87,共4页
Food Science
