摘要
目的建立用新型四唑氮盐MTS和XTT做比色分析测定淋巴细胞增殖效应的方法。方法分别用MTS、XTT结合PMS,对小鼠脾淋巴细胞密度和增殖进行比色测定,选择最佳实验条件,并将这两种方法与3H-TdR掺入法和MTT法比较。结果用MTS和XTT比色分析法测定小鼠脾淋巴细胞的增殖,在0.5～4×109/L的范围内,所获光吸收(A)值与细胞密度呈良好的相关性。最佳细胞密度为2×109/L,最佳反应时间为6h,最佳PMS的终浓度为1×10-4mol/L。两种比色法与3H-TdR掺入法的结果相一致,且两种新方法较MTT法灵敏。结论MTS和XTT比色分析法可代替3H-TdR掺入法和MTT法,用于测定淋巴细胞的增殖效应。
Aim To develop a new method for assessing lymphocytic proliferation effect by new tetrazolium salt MTS and XTT colorimetric assays. Methods Murine splenic lymphocyte density and proliferation were evaluated by MTS, XTT combined PMS respectively. The optimal experimental conditions were selected and the two methods were compared with 3H TdR incorporation and MTT assays. Results There was a good correlativity between optical absorbance(A) value and cell densities ranged 0.5～4×109/L when assaying murine splenic lymphocyte proliferation by MTS and XTT colorimetric assays. The optimal cell density, reactive time and final PMS concentration were 2×109/L, 6 h and 1×10 4mol/L,respectively. The results of two colometric assays corresponded with that of 3H TdR incorporation and the two new methods were more sensitive than MTT assay. Conclusion MTS and XTT colorimetric assay may be well used to assess lymphocytic proliferation effect.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2001年第3期292-295,共4页
Chinese Journal of Cellular and Molecular Immunology
