摘要
借鉴高分子亚浓溶液线团收缩理论 ,研究了脱氧核糖核酸 (DNA)片段在高分子溶液全浓度区间的电泳迁移行为。结果表明 ,在高分子稀溶液、亚浓溶液和浓溶液 3个不同浓度区间 ,DNA的电泳迁移行为各不同 ,DNA片段的分离在这 3个浓度区间也存在差异。
The theory of polymer coils shrinking in semi dilute solution has recently been developed on polymer solution The polymer solution from coils shrinking concentration C S to uniform entangled concentration C + has been defined as semi dilute solution We experimentally investigated the electrophoretic migration behavior of 100 bp deoxyribonucleic acid (DNA) Ladder in hydroxypropyl methyl cellulose (HPMC) concentration ranging from 1 25 g/L to 16 06 g/L The friction force mobility μ f is used to express the friction force that DNA will encounter in capillary electrophoresis Our results indicate the division of polymer solution into three regions depending on the relationship of μ f and HPMC concentration and Ferguson plot Resolutions of 200 bp/300 bp and 700 bp/800 bp show semi dilute polymer solution suits large fragments and small fragments DNA separation respectively The results confirm that the current polymer theory is valid under actual CE condition
出处
《色谱》
CAS
CSCD
北大核心
2001年第1期60-63,共4页
Chinese Journal of Chromatography
