摘要
目的 探讨“一肾一夹 (1k1c)”高血压大鼠动脉平滑肌细胞钠泵α亚单位各异构体基因表达的改变 ,为高血压发病机制的深入研究提供理论及实验依据。方法 分别应用分子生物学RT PCR及免疫组化技术 ,探讨 1k1c高血压大鼠动脉平滑肌细胞钠泵α1、α2及α3亚单位mRNA及蛋白水平基因表达的改变。结果 1k1c高血压大鼠动脉平滑肌细胞钠泵α亚单位基因表达发生改变 :无论在mRNA或蛋白水平 ,α1亚单位表达增强 ,而α2与α3亚单位表达无改变。结论 1k1c高血压大鼠动脉平滑肌细胞钠泵α亚单位基因表达发生改变 ,这种改变可能参与了该模型的血压升高机制。
Objective\ To investigate the gene expression of sodium pump α\|subunit in aortic smooth muscle of 1\|kidney\|1\|clip (1k1c) hypertensive rats. Methods\ 1k1c hypertensive rats were prepared by partially ligating the left renal artery and removing the right kidney. 4 weeks later, all the rats were killed and sodium pump α1\|, α2\|, and α3\|subunit in aortic smooth muscles were detected with reverse transcription polymerase chain reaction (RT\|PCR) method and immunohistochemical assay at both mRNA and protein levels, respectively. Resulsts\ Changes in the expression of sodium pump α\|subunit gene were found in aortic smooth muscles of 1k1c hypertensive rats: α1\|subunit increased at both mRNA protein levels, while α2\| and α3\|subunits remained without changes. Conclusions\ There were great changes in the gene expression of sodium pump α\|subunit in aortic smooth muscles of 1k1c hypertensive rats, which might be related to the development of hypertension in this hypertensive model.
出处
《高血压杂志》
CAS
CSCD
2001年第2期140-142,共3页
Chinese Journal of Hypertension
