摘要
实验以不同季节的茎尖 (含茎段 )为材料 ,采用不同基本培养基 ,研究确定西洋杜鹃组织培养适合的基本培养基类型 ,植株再生的可能途径 ,以及适宜的培养条件。结果表明 :①低盐分浓度及高比值NH4 +/NO3 -的基本培养基Read培养基适合西洋杜鹃 ;②最适外植体为摘花芽后萌发的顶芽茎尖 ;③最佳取材时间为 3～ 5月 ;④培养条件 :温度 2 5± 2℃ ,光照 16h/黑暗 8h ,光照强度 15 0 0～ 2 5 0 0lx。
In view of studying the suitable type of medium, probable way of explant regeneration and suitable condition of culture for tissue culture in R.hybridn, a experimentation was established in which shoot tip from different seasons and a series of different medium were employed. The results are as follows: ①Read medium with low mineral salt concentration and high ration of NH 4 +/NO 3 - is suitable for R.hybridn microculture; ②The optimal explants are shoot tips of end buds excised from R.hybridn in 3～5 months; ③All the cultures are kept under the temperature of 25±2℃, illuminated for 16 hours per day, and light intensity was 1500～2500lx.
出处
《四川农业大学学报》
CSCD
2001年第1期37-39,共3页
Journal of Sichuan Agricultural University
