摘要
以培矮 64S和盐恢 559的成熟胚和未成熟胚为外植体 ,研究对 2个籼稻品种的组织培养效果的影响因素 .在诱导培养过程中 ,2个品种对最适 2 ,4二氯苯氧乙酸 ( 2 ,4 D)浓度要求不一样 .在适宜的2 ,4 D浓度的基础上 ,添加某些外源激素 ,并不能提高对愈伤组织的诱导效果 .在继代过程中 ,交替使用不同浓度的 2 ,4 D,能明显改善愈伤组织的生长状态 ,提高愈伤的质量 .对不易形成胚性愈伤组织的品种(盐恢 559) ,在继代过程中加入适量的脱落酸 ,可明显改善愈伤组织的生长状态 ,增加胚性愈伤组织的数目 .不同糖类试验结果表明 :麦芽糖较蔗糖更适宜作为籼稻的碳源 ,综合考虑成本和效率问题 。
Factors affecting the tissue culture were studied in two Indica rice cultivars, Pei′ai64S and YanHui559. The results showed that the suitable concentration of 2,4D to induce rice calli was different between cultivars. With the most suitable concentration of 2,4D and other exohormone, such as KT, did not improve callusing. However, application of different concentration of 2,4D alternatively could improve the quality of calli in terms of the growth state of subcultured calli, meanwhile, applying certain amount of ABA to the subculture medium could improve the growth state of calli. Experiment on different kinds of carbohydrate showed that maltose was more suitable as the source of carbon than sucrose. In order to reduce cost and raise efficiency, other alternative kinds of carbohydrate or different carbohydrates should be used mixedly.
出处
《扬州大学学报（自然科学版）》
CAS
CSCD
2001年第2期37-41,共5页
Journal of Yangzhou University：Natural Science Edition
基金
国家高新技术发展计划项目!(86 3 10 10 10 2 0 1)
