摘要
采用逆流萃取与反相高效液相色谱相结合的方法 ,富集并测定了川芎中川芎嗪的含量。色谱柱 :Zorbax ODS柱 ,流动相 :甲醇 :水 (1%醋酸 ) =35 :6 5 ,流速 :0 .8m l/min,检测波长 :30 0 nm,线性范围 :0 .41μg/m l～ 4.1μg/m l,r=0 .9993,回收率 :92 .3%。方法简便、快速、灵敏。用此法对购自不同地区的川芎进行了分析 ,结果提示
An analytical method for determination of tetramethylpyrazine (TMP) in ligusticum chuanxiong hort by RP HPLC coupled with countercurrent extraction used for the preconcentration of trace TMP was established. The column was packed with Zorbax ODS (250 mm×4 mm, 5 μm). The mobile phase consisted of methanol water (1 % acetic acid)(35∶65), the flow rate was 0.8 ml/min and detection was performed at 300 nm. The calibration curves showed good linearity over the range of 0 41 μg/ml to 4 1 μg/ml, γ=0 9993, the recoveries were 92 3 %. The method was simple, fast, sensitive and accurate and could be applied to the analysis of ligusticum chuanxiong hort. The results showed that the content of TMP in ligusticum chuanxionghort were different with different sources.
出处
《同济医科大学学报》
CAS
CSCD
北大核心
2001年第3期209-210,共2页
Acta Universitatis Medicinae Tongji
关键词
川芎
川芎嗪
反相高效液相色谱法
逆流萃取
ligusticum chuanxiong hort
tetramethylpyrazine
RP HPLC
countercurrent extraction
