摘要
从活性污泥里筛选到一株巨大芽孢杆菌 (Bacillusmegaterium)A2 5,它能分泌胞外絮凝剂。用乙醇沉淀及SephadexS 50 0分子筛层析得到絮凝剂纯品BP2 5。通过Bradford反应、琼脂糖凝胶电泳及硫酸 酚法测定糖 ,证明BP2 5是一类多糖类物质。应用核磁共振技术证明其不含有糖醛酸。经三甲基硅醚和甲基化的气相色谱 质谱分析 ,证明多糖BP2 5含有葡萄糖和甘露糖两种单糖 ,其摩尔比为 4∶1。其连接键型包括α 1 ,6糖苷键和α 1 ,3糖苷键。由此推导出BP2 5可能的单元结构。
A strain of Bacillus megaterium screened from activated sludge could produce exocellular flocculant.The flocculant was purified through ethanol precipitation and Sephadex S 500 column chromatography.The purified flocculant BP25 was assayed by Bradford reaction,agarose gel electrophoresis and Sulfate\|phenol method.The results showed that BP25 was a kind of polysaccharide which contains 36.97% O,6.28% H,47.00% C,0% N\.NMR assay showed BP 25 contains no uronic acid. Gas chromatography assay combined with thin\|layer chromatography of acid hydralate revealed that BP25 contains Glucose and Mannose with the mole ratio of 4∶1.Methylation analysis revealed the polysaccharide contains α 1,6 glycosidicbond and α 1,3 glycosidicbond.The main chains are comprised of Glucose and all the Mannose are in the side chains.Possible repeating unit structure was deduced.
出处
《微生物学报》
CAS
CSCD
北大核心
2001年第3期348-352,共5页
Acta Microbiologica Sinica
基金
国家"九五"攻关项目 ( 96 90 9 0 1 0 3 )&&
