摘要
目的 探讨从成年新西兰兔坐骨神经分离培养获得大量雪旺细胞的有效手段。方法 利用成年兔发生Wallerian变性的坐骨神经用植块法进行培养 ,通过相差显微镜活细胞计数和S 10 0细胞化学标记相结合鉴定了雪旺细胞增值和纯化程度。结果 通过上述方法获得大量雪旺细胞纯度达 95 % ,并绘制其生长曲线 ,体外培养的雪旺细胞倍增时间为 8d。结论 本方法能获得大量高纯度的雪旺细胞 。
Purpose: To present an effective technique for culture and expansion of Schwann cells (SC) from adult rabit peripheral neres. Methods: Adult predegenerated rabbit sciatic nerves culture in defined medium by primary cultures of adult SC. the purity of SC was identified through the phase contrast microscope and S-100 immunocytochemical staining. Results: The best results were obtained with a conditioned medium supplemented with 20% fetal calf serum. In these conditions the purity of SC was about 95% in vitro. The growth curve was drawed. The doubling time of the 3rd passage of cells was 8 days. Conclusions: These findings indicate that adult SC can be expanded from small preinjured nerve fragments in a short time period to provide a source of SC for tissue engineering cellular transplants.
出处
《复旦学报（医学版）》
EI
CAS
CSCD
北大核心
2001年第3期230-232,F003,共4页
Fudan University Journal of Medical Sciences
