摘要
目的 :研究喉癌 DNA倍体、细胞周期特征及超微结构 ,并分析其临床意义。方法 :采用流式细胞术(FCM)检测 30例冻存的喉癌标本及 5例声带息肉患者术中修复的喉部组织 ,同时观察其超微结构。结果 :喉癌标本与喉部粘膜的异倍体率分别为 6 0 .0 % (18/ 30 )、 G0 ,S期百分比分别为 2 0 .33± 6 .12及 12 .35± 3.81,DI值分别为 1.78± 0 .82及 1.0 7± 0 .0 4,PI值为 2 9.0 9± 6 .8及 11.75± 3.75。喉癌标本的异倍体率 ,DI值 ,PI值 ,S期的百分比明显高于正常组织 (P <0 .0 5 )。光镜下发现异倍体肿瘤分化差 ,核分裂相多 ,二倍体肿瘤分化好。电镜下发现异倍体肿瘤的核大 ,深染 ,畸形明显。结论 :喉癌的异倍体率与肿瘤的位置、细胞分化有关 ,流式细胞术在揭示喉癌的肿瘤生物学行为及指导临床治疗方面有重要的意义。
Objective: To detecte the DNA pliody, cell cycle character and ultrastructure in the tissue of laryngeal squamous cell carcinoma for analyzing their clinical significance. Methods: The detection of DNA pliody by flow cytometry in the tissue of laryngeal squamous cell carcinoma on 30 specimens and 5 samples of normal laryngeal tissue. Results: The DNA aneuploidy (AN) rate was 60 0% (18/30) and 0 0 % (0/5) in laryngeal carcinoma and normal laryngeal tissue respectively. In group of laryngeal carcinoma, the rate of S phase, DNA index (DI) nd DNA proliferation index (PI) were 20 33±6 12, 1 78±0 82, and 29 09±6 89, respectively; in group of normal laryngeal tissue, there were 12 35±3 81, 1 07±0 04, and 11 75± 3 75 respectively. Those results of DI, PI and S phase in group of laryngeal carcinoma showed higher than that in normal subjects (\%P\%<0 05). The ultrastructure of AN tumor showed poor differential than that of dipliody tumor. Conclusion: The AN rate of laryngeal carcinoma was closely related with site and differentiation, and the detection of DNA contents had significance in evaluating tumor biologic behavior.
出处
《耳鼻咽喉（头颈外科）》
2001年第3期169-169,共1页
Chinese Arch Otolaryngology-Head Neck Surg
