摘要
近年来 ,一些不依赖于转录因子活性的新型双杂交系统相继建立 ,如分离的泛素系统、蛋白质片段互补分析、阻遏物重构分析和SOS恢复系统等。同利用转录因子活性的酵母双杂交系统相似 ,这些方法也利用了一些活性蛋白的结构与功能特点来研究蛋白质间相互作用 ,这些活性蛋白不是转录因子 ,但也可在结构上进行分离并可通过重构使其生物活性得以恢复。由于这些新型双杂交系统的各自特点 ,使得它们成为酵母双杂交系统的有益补充和研究蛋白质间相互作用的有力工具。
Some new type of two-hybrid systems,such as split-ubiquitin system, protein-fragment complementation assay, repressor reconstitution assay and SOS recruitment system, have been developed recently. Similar to the original transcription-based yeast two-hybrid system, these systems are to establish an assay for protein-protein interactions, in which some functional proteins can be split into two parts in structure and their activities can be recovered by reconstitution. Due to their non-transcriptional properties, these new types of two-hybrid systems have become useful extension of the yeast two-hybrid system and powerful tools for the study of protein interactions.
出处
《生理科学进展》
CAS
CSCD
北大核心
2001年第3期229-232,共4页
Progress in Physiological Sciences
基金
国家自然科学基金 (3990 0 182 )资助课题
关键词
双杂交
蛋白质间相互作用
融合蛋白
Two-hybrid
Protein-protein interaction
Fusion protein
