摘要
通过对一株粪产碱杆菌WX C12 (Alcaligenesfaecalis)的摇瓶发酵研究 ,确定了其最佳发酵培养基组成及最适摇瓶发酵工艺条件 .摇瓶实验确定的最佳培养基组成为 ( g/L) :葡萄糖 50 ,NH4 Cl 1.0 7,KH2 PO4 2 .72 ,K2 HPO4 1.71,MgSO4 ·7H2 O 0 .4 9,酵母膏 1,少量无机盐溶液 .最佳工艺条件为 :初始 pH 7.0 ,装液量为 60mL/ 2 50mL摇瓶 ,摇床转速为 2 2 0r/min ,温度为 30℃ .15L自动机械搅拌罐放大试验结果表明 ,在不改变操作参数的情况下 ,经 60h发酵 ,热凝胶产量可达2 .2 % ,转化率超过 50 %
With a strain of Alcaligenes faecalis WX C12, the optimal medium and culture condition for curdlan production was investigated. The optimal medium was established as follows (g/L): glucose 50; NH 4Cl 1.07; KH 2PO 4 2.72; K 2HPO 4 1.71; MgSO 4·7H 2O 0.49; Yeast extract 1 and trace amount of salt solution. The optimal culture conditions were that: initial pH 7.0, volume 60 mL/250 mL, shaking speed 200 r/min, temperature 30 ℃. With this medium, batch culture was conducted in a 15 L fermentor equipped with sensing/control devices. After 60 hours fermentation, the production and yield of curdlan was 22 g/L and 50%, respectively.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2001年第4期347-350,共4页
Journal of Wuxi University of Light Industry
基金
江苏省科技攻关 (国际合作 )项目 (BS99719)资助课题
