摘要
目的 研究钙离子通道拮抗剂硝苯地平对人牙龈成纤维细胞的影响。方法 以硝苯地平 12 0 0 0、36 0 0、10 8 0和 32 4μg/L作体外培养牙龈成纤维细胞细胞计数观察。并以3 H 胸腺嘧啶脱氧核苷 (3 H TDR)细胞内掺入法评价DNA合成量。结果 细胞计数在实验的第 6、7d组间差异有极显著性。主要表现为硝苯地平 12 0 0 0 μg/L和 36 0 0 μg/L组 ,细胞计数明显高于低浓度组。各组间DNA合成量差异有极显著性。进一步分析差异存在于高浓度组与其他各组之间。
Objective To investigate the effects of nifedipine, a calcium channel blocker, on cellular proliferation in gingival fibroblasts in vitro. Methods Gingival fibroblasts were cultured and incubated with various concentrations of nifedipine (1 200 0, 360 0,108 0 and 32 4 μg/L) for up to 7 days. 3H TdR incorporation assay in vitro taken was used to evaluate the amount of DNA synthesis. Results Cell numbers were significantly different among these groups on sixth and seventh day, especially higher in 1 200 0 μg/L and 360 0 μg/L groups. The incorporation percentage in high concentration groups were significantly higher than those in others. Conclusion Nifedipine can influent proliferation of gingival fibroblsts in high concentration.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2001年第3期180-182,共3页
Chinese Journal of Stomatology
基金
江苏省自然科学基金资助项目 (BJ990 68)
