摘要
对分离出的一株凝结芽孢杆菌生产糖脂的摇瓶发酵工艺进行了优化和 1 0L罐发酵实验。适宜发酵条件为 :培养基由 6 %豆油、 3 5g/LNaNO3、 0 75g/L酵母膏以及一定量的无机盐组成 ,发酵温度 3 0℃ ,初始pH8 5 ,搅拌转数 1 5 0~ 2 4 0r/min ,发酵周期 96h。糖脂产量达到 7 0 73g/L。
The biotechnology of glycolipid fermented b y a bacillus coagulans was studied and the fermentation pro cess in 10L bioreactor was conducted.Suitable medium contained 6% bean oil as ca rbon source,3 5g/L NaNO 3 as nitrogen source,0 75g/L yeast extract and some i no rganic salts.The fermentation temperature of 30℃,initial pH of 8 5,strring rev olution of 150~240r/min and fermentation period of 96h proved to be optimal.The yield of glycolipid in 10L bioreactor was 7 073g/L.
出处
《微生物学通报》
CAS
CSCD
北大核心
2001年第4期12-15,共4页
Microbiology China
基金
黑龙江省教委资助项目