摘要
目的 探讨胃癌细胞p16蛋白表达情况及其与DNA含量的相关性及意义。方法 应用流式细胞仪 (FCM)和图像分析仪 (ICM)检测 41例胃癌细胞p16蛋白表达量和DNA含量 ,10例正常胃组织也用FCM测p16蛋白表达量。结果 41例胃癌p16蛋白表达量明显低于正常胃组织 (荧光指数分别为 0 .81± 0 .0 9和 1.0 0± 0 .11,P <0 .0 1) ,p16蛋白表达量与胃癌病理分级有关 ,随病理分级增高而降低 (P <0 .0 5 ) ,与胃癌患者的性别、年龄无关。胃癌DNA相对倍体均值 (U值 )范围为 1.14～2 98,U值随病理分级增高而升高 (P <0 .0 1)。同时 ,胃癌p16蛋白表达量与DNA含量呈负的直线相关 (P <0 .0 1)。结论 p16蛋白的异常表达与胃癌的发生发展、恶性程度有关 ,并具有较重要临床意义。
Objective To investigate p16 protein expression and its correlation with DNA quantity in gastric carcinoma (GC) cells. Methods p16 protein expression was quantitatively determined by flow cytometry (FCM) in 41 cases of GC and 10 cases of normal gastric tissues. DNA quantitative analysis of 41 cases of GC was performed by image cytometry (ICM). Results p16 protein expression in GC was significantly lower than that in normal gastric tissues (respective FI: 0.81± 0.09 and 1.00±0.11, P<0.01). The expression of p16 protein did not show correlation with such factors as sex and age in GC, but it was significantly stronger in well differentiated GC than that in poorly differentiated GC (P<0.05). The mean of DNA relative ploid (U value) in GC was from 1.14 to 2.98. U value becomes higher with the increase in grading (P<0.01). There was significant negative linear correlation between p16 protein expression and U value in GC (P<0.01). Conclusion Abnormal expression of p16 protein is closely correlated to the occurrence, development and malignancy degree of GC. The level of p16 protein expression implies important clinical significance for GC treatment and may serve as an indicator to predict the prognosis of patients.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2001年第3期191-193,共3页
Chinese Journal of Pathology
