摘要
对海南槟榔黄化病病株组织进行超薄切片电子显微镜观察和四环素族抗菌素注射诊断，结果表明：海南槟榔黄化病病株的叶脉、叶鞘基部呈水渍状的幼嫩花苞组织内的筛管细胞和伴胞部位均发现植原体（Ｐｈｙｔｏｐｌａｓ ｍａｓ）。植原体的大小为 １８０～５５０ｎｍ，单位膜厚度为９～１３ｎｍ。但在健康的槟榔植株的相应组织中未发现上述病菌。经四环素族抗菌素２种药物注射槟榔黄化病病株均可不同程度地抑制病情发展。上述２种鉴定符合对植原体病原的鉴定程序，故可进一步证实植原体（Ｐｈｙｔｏｐｌａｓｍａｓ）是引起海南槟榔黄化病的一种病原。
Examined under electronic microscope, Phytoplasmas was found in the sieve cells and the companion cells of the leaf vein tissues and the tender bud tissues from arecanut palm (Areca catechu L.) infected with the yellow leaf disease. The size of the Phytoplasmas was 180-550 nm, with one layer of membrane 9-13 nm thick. These Phytoplasmas were not found in the corresponding tissues from the healthy arecanut palms. The arecanut palms affected by the yellow leaf disease could convalesce after injected with Doxycyclin Hyclate and Oxytetracycline Hydrochloride. It was confirmed that Phytoplasmas was a kind of pathogens causing yellow leaf disease of arecanut in Hainan Island.
出处
《热带作物学报》
CSCD
2001年第2期43-46,共4页
Chinese Journal of Tropical Crops
基金
国家自然科学基金资助项目
