摘要
建立了反相银化高效液相色谱测定银杏叶中银杏酚酸含量的分析方法。在样品的浸提液中加入少量酸性盐溶液和吸附剂后 ,用一步反萃取法净化样品 ,有机相浓缩后供HPLC分析。流动相 :V(甲醇 )∶V(体积分数为 5 %的乙酸水溶液 ) =90∶10 ,其中银离子浓度 0 0 3mol·L-1,紫外检测波长 310nm。结果表明 4种银杏酚酸之间达到基线分离 ,该方法平均回收率为97 3% ,相对标准偏差 1 6 % ,最低检测量 0 0 2 6 μg ,可有效地用于银杏叶及其提取物中银杏酚酸的定量分析。
An analytical method has been firstly achieved for the quantification of ginkgolic acids from Ginkgo biloba leaves by reversed phase argentation high performance liquid chromatography. Analytical sample was cleaned up after addition of acidic salt solution and adsorbent to the matrix solution by counter extraction of analytes with hexane. Ginkgolic acids were determined by HPLC with methanol and 5% aqueous acetic acid (90∶10, V/V ) containing 0.03 mol·L -1 silver ion as mobile phase and UV detection at 310 nm. Results showed ginkgolic acids were separated successfully from each other and from other interfering components, which were confirmed by spectra analysis and purity assay. The linearity of the calibration curve was good in the range of 0.084 μg 10.56 μg ( r = 0 999?8 ). The average recovery was 97.3% and RSD was 1.6%. The detection limit was 0.026 μg ( S/N =3). The convenient method can be used as a reliable tool for the quantitative analysis of ginkgolic acids.
出处
《色谱》
CAS
CSCD
北大核心
2001年第3期207-210,共4页
Chinese Journal of Chromatography
关键词
反相银化高效液相色谱法
定量分析
银杏叶
银杏酚酸
植物药
reversed phase argentation high performance liquid chromatography
quantitative analysis
Ginkgo biloba leaves
ginkgolic acids
