摘要
目的 :研究康复训练对实验性脑梗塞偏瘫大鼠行为学和脊髓生长相关蛋白 (GAP - 43)表达的影响。方法 :采用光化学法制成脑梗塞偏瘫大鼠模型 ,并随意分为康复训练组和制动组 ,每天置于滚筒式网状训练器内转动训练 ,在平衡木、转棒上做行走训练 ;共 30min ;每周 5次。在梗后 2 4h、7d、14d、2 1d、2 8d观察行为学变化和脊髓的GAP- 43的表达 ,同时以正常大鼠作为对照。结果 :Bederson神经功能、平衡木、转棒行走 (7- 2 8d)、网屏实验 (7- 14d)康复组较制动组明显好转 (P <0 .0 5 )。正常组脊髓的GAP - 43较少 ;脑梗塞后 2 4h康复组、制动组大鼠脊髓未瘫痪侧脊髓前角有大量的GAP - 43的表达 ,脑梗塞后 7- 14d康复组瘫痪侧及未瘫痪侧脊髓前角区均有GAP - 43表达 ,制动组阳性染色淡 ;2 1- 2 8d康复组未瘫痪侧脊髓前角较瘫痪侧GAP - 43表达的多 ,而制动组不明显。结论 :脑梗塞后康复训练可促进行为恢复及脊髓前角GAP -
Objective: To study the effect of rehabilitative training on behavioral recovery and expression of GAP-43 in spinal cord after cerebral infarction rats. Method: The cerebral infarction rats model were established by photochemical injury and divided into rehabilitative training group and braking group. The behavior and expression of GAP-43 in spinal cord of the experimental and normal rats were investigated at 24h、7d、14d、21d and 28d after cerebral infarction. Result and conclusion: Bederson neural function, balancing, rotating walking function (7-28d) and mesh exercise (7-14d) in rehabilitation group rats were recovered better than those of the braking group. Expression of GAP-43 in spinal cord of normal rats was not remarkable. In spinal cord anterior horn of non-paralysis side in rehabilitation training and braking group many GAP-43 positive reaction were observed at 24h after cerebral infarction. After 7-14d of cerebral infarction, on bilateral spinal cord anterior horn of rehabilitation group rats GAP-43 positive reaction were observed. GAP-43 positive reaction were insignificant and mild in braking group rats. After 21-28d of cerebral infarction, expression of GAP-43 in paralized side of rehabilitation group rats were more intensive than non-paralized side of rehabilitation group and braking group.
出处
《中国康复医学杂志》
CAS
CSCD
2001年第3期132-136,共5页
Chinese Journal of Rehabilitation Medicine
基金
军队留学人员回国启动基金 !NO .97卫科训字 116号回国基金
