期刊文献+

核酸扩增检测在血液筛查的初步应用 被引量:47

Nucleic acid amplification test of blood donations in blood bank
原文传递
导出
摘要 目的 为提高血站供血安全性 ,探讨核酸扩增检测在血站血液筛查中的可行性。方法 在酶联免疫吸附试验 (ELISA)常规筛查血液的基础上 ,采用荧光定量聚合酶链反应 (PCR)方法 ,检测乙型肝炎表面抗原 (HBsAg)、丙型肝炎抗体 (抗 HCV)和人免疫缺陷病毒抗体 (抗 HIV) 1/ 2呈阴性的献血者微量血浆汇集池标本 (2 0人份× 5 0 μl汇集 )中的丙肝病毒 (HCV)和乙肝病毒 (HBV)核酸 ,再对阳性汇集池中的标本进行单份检测。结果  880 5份 (分 44 2个汇集池 )血液被检测HCVRNA ,结果有1例 (0 .0 1% )为阳性 ;144 1份血液被检测HBVDNA ,结果 6例 (0 .4% )为阳性。从标本汇集到筛查出单份阳性献血者大约需要 3d。结论 ELISA结合荧光定量PCR检测微量血浆汇集池标本的方法筛查血液HBV和HCV感染是可行的 。 Objectives To increase the safety of blood supply and to evaluate the feasibility of Nucleic Acid amplification test (NAT) of blood donations in blood bank. Methods Individual donor plasma samples serologically negative for HCV, HBV and HIV detected by ELISA were pooled according to the size of 20×50 μl. HCV RNA and HBV DNA in pooled samples were detected by AcuGen AG 9600 AmpliSensor qualitative PCR methods. Individual donor plasma samples in positive pooled samples were further tested by PCR. Results One(0.01%)of 8805 donations was PCR for HCV RNA positive. Six (0.4%)of 1 441 donations were PCR for HBV DNA positive. The whole procedure took three days from pooling donor plasma samples to identifying the positive samples. Conclusion It is feasible to incorporate NAT into ELISA screening blood donations for HBV and HCV. NAT will further increase the safety of blood supply.
出处 《中华检验医学杂志》 CAS CSCD 北大核心 2001年第3期141-143,共3页 Chinese Journal of Laboratory Medicine
关键词 乙型肝炎病毒 C型肝炎样病毒属 供血者 基因扩增 血液筛查 ELISA PCR Hepatitis B virus Hepatitis C like viruses Blood donors Gene amplification
  • 相关文献

参考文献6

  • 1Busch MP,Rawal BD,Nowicki M,et al.HCV RNA and ALT patterns in seroconverting transfusion recipients and correlation with donor ALT and RNA[].Transfusion.1997
  • 2Vrielink H,Reesink HW,van den Burg PJM,et al.Performance of three generations of anti-hepatitis C virus ELISA in donors and patients[].Transfusion.1997
  • 3Schottstedt V,Tuma W,Bunger G,et al.PCR for HBV, HCV and HIV-1 experiences and first results from a routine screening programme in a large blood transfusion service[].Biologicals.1998
  • 4Schreiber GB,Busch MP,Kleinman SH,et al.The risk of transfusiontransmitted viral infection[].The New England Journal of Medicine.1996
  • 5Roth WK,Weber M,Seifried E.Feasibility and efficacy of routine PCR screening of blood donations for hepatitis C virus, hepatitis B virus, and HIV-1 in a blood-bank setting[].The Lancet.1999
  • 6Cardoso MS,Koerner K,Kubanek B,et al.Mini-pool screening by nucleic acid testing for hepatitis B virus, hepatitis C virus, and HIV: preliminary results[].Transfusion.1998

同被引文献389

引证文献47

二级引证文献441

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部