摘要
目的 :研究驻极体对成纤维细胞表面电荷的影响及其细胞表面电荷与细胞生长、细胞凋亡的关系。方法 :选用± 30 0V和± 10 0 0 V驻极体分别作用成纤维细胞 2 4、48和 72 h,用流式细胞术和细胞电泳技术测定成纤维细胞的生长周期和细胞表面电荷。结果 :(1)负极性驻极体作用成纤维细胞使细胞的电泳率和 S期内的细胞数显著增加。 (2 )成纤维细胞表面负电荷和 S期内细胞数的增加量与驻极体的表面电位成正比。 (3)负极性驻极体能减少凋亡细胞的表面电荷量。 (4)正极性驻极体作用成纤维细胞使 G2 和 S期中的细胞数减少 ,同时减少了细胞表面的负电荷数量。结论 :负极性驻极体具有促进成纤维细胞生长和增加细胞表面负电荷量的作用。正极性驻极体具有抑制成纤维细胞生长和降低细胞表面电荷密度的作用。凋亡细胞的表面电荷量较正常细胞要少。
Objective: To study the influence of electret on surface charge of fibroblast cells (3T3 cells) and to probe the relationship between cell growth, apoptosis and cell surface charge. Methods: Electrets Teflon PTFE, ±300 V,±1 000 V were used to treat 3T3 cells for 24, 48 and 72 h. Then the influences of electrets on cell cycle and surface charge of 3T3 cells were studied by flow cytometry and electrophoresis, respectively. Results: (1) After 24 h action of negative electrets, electrophoretic mobility (or surface charge) and cell number in S phase of 3T3 cells were significantly increased compared with those in control group. (2) Effect of negative electrets enhancing cell growth and increasing cell surface charge was in proportional to the surface potential of electret. (3) Surface charge density of apoptotic cell was reduced by electret. (4) After 24 h action of positive electret, the cell number in S and G 2 phase were decreased and cell surface charge was also reduced. Conclusion: Negative electret can improve cell growth and increase cell surface charge density. Positive electret can restrain cell growth and reduce cell surface charge density. Surface charge of apoptotic cell is less than that of normal cell.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2001年第5期405-408,共4页
Academic Journal of Second Military Medical University
基金
This project is supported by National Natural Sci-ence Foundation of China( No.3 9770 3 69) .
