摘要
目的 :建立 1 甲基 4苯基吡啶离子 (1 methyl 4 phenylpyridiniumion ,MPP+)诱导的大鼠小脑颗粒细胞凋亡模型 ,探讨肉苁蓉成分是否对MPP+诱导的大鼠小脑颗粒细胞凋亡有保护作用。方法 :用肉苁蓉成分预先孵育大鼠小脑颗粒细胞 ,经MPP+处理这些细胞后 ,用甲基绿 派诺宁染色、DNA凝胶电泳、流式细胞术检测。结果 :浓度为 5 0 μmol·L-1MPP+使小脑颗粒细胞发生典型的凋亡 ,2 5mg·L-1campneosideⅡ具有抗凋亡作用。结论 :以MPP+为诱导剂建立的大鼠小脑颗粒细胞凋亡模型 ,可用于研究与帕金森病 (Parkinson’sdisease ,PD)有关的细胞凋亡的调控机制和筛选抗PD药物 。
To establish an apoptosis model induced by 1 methyl 4 phenylpyridinium ion (MPP +) in Cerebellar granule neurons (CGNs) and assessed the effect of campneoside Ⅱ which is a compound contained in Cistanche tubulosa (Schenk) R. Wight. Methods: CGNs were treated with MPP + and then staining with methyl green and pyronin, internucleosomal DNA degradation with agarose gel electrophoresis analysis and flow cytometry of neuronal apoptosis were performed . Results: Treatment of 50 μmol·L -1 MPP + for 24 h or longer induced an initiated apoptosis in CGNs and the latter showed typical morphological features, including condensed chromatin, nuclear fragmentation and apoptotic body. Agarose gel electrophoresis of DNA from the cells treated with MPP + revealed “ladder” pattern and a time dependent increase of apoptotic peak by flowcytometric analysis. Treatment of 25 mg·L -1 campneoside Ⅱ could protect cells from apoptosis induced by MPP +. The degraded fragments of DNA decreased obviously. The viability percentage of cells was improved within 24 h. Conclusion: A new and simple method of quantitating MPP + induced apoptosis in CGNs was established. The campneoside Ⅱ inhibited MPP + induced apoptosis and the DNA fragmentation. This method can be used for studying apoptosis mechanism involved in the pathogenesis of Parkinson disease and screening anti Parkinson's disease drugs.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2001年第3期217-220,共4页
Journal of Peking University:Health Sciences
基金
国家重点基础性研究 973项目基金!(G19980 5 112 4)资助&&
关键词
1-甲基-4-苯基吡啶离子
肉苁蓉
药理学
神经毒素
细胞凋亡
药物作用
methyl 4 phenylpyridinium ion (MPP +)
Campneoside Ⅱ
Cistanche tubulosa (Schenk) R. Wight/pharmacol
Neurotoxins
Apoptosis/drug eff
