摘要
目的 探讨高糖对系膜细胞蛋白激酶Ｃ（ＰＫＣ）活性的影响及ＰＫＣ在系膜细胞增殖、细胞外基质积聚中的作用。方法 采用大鼠系膜细胞进行体外培养，高糖作为激动剂，佛波酯（ＰＭＡ）作为ＰＫＣ抑制剂，甘露醇作为渗透压对照，用液闪仪测定ＰＫＣ活性，３Ｈ－ＴｄＲ掺入法检测细胞增殖，ＥＵＳＡ法测定培养上清中纤维连接蛋白（ＦＮ）含量。结果 高糖可增加系膜细胞颗粒部分ＰＫＣ活性。抑制细胞增殖、促进ＦＮ分泌，且与渗透压无关。抑制ＰＫＣ后，可阻止高糖诱导的ＦＮ分泌。结论 高糖可激活系膜细胞ＰＫＣ，促进细胞外基质积聚和糖尿病肾病的发生。
Objective To investigate the activity and possible role of protein kinase C (PKC) in mesangial cells in response to high glucose. Methods Rat mesangial cells were cultured and the PKC activity was measured by liquid scintillation counting. 3H-thymidine incorporation was used to assess cellular proliferation. Production of fibronectin( FN) was analysed by ELISA. Results Compared to normal glucose, high glucose increased the activity of PKC and the production of EN, and decreased 3H-TdR incorporation. Inhibition of PKC with phorbol ester for 24 hours blocked the increase of FN but had no effect on cellular proliferation under high glucose medium. Conclusion High glucose can activate PKC in mesangial cells and contribute to deposition of extracelluar matrix of diabetic nephropathy.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
2001年第3期168-170,共3页
Chinese Journal of Nephrology
基金
国家自然科学基金资助项目(39870297)
