摘要
借鉴哺乳动物内皮细胞体外培养技术 ,结合鱼类细胞自身特点 ,纯化培养鲤血管内皮细胞并传至 3代 ,以此作为粘附材料。应用淋巴细胞分离液离心技术并结合玻璃粘附法分离纯化鲫、草鱼外周血中淋巴细胞、单核细胞 ,并与上述 3代内皮细胞进行免疫粘附试验 ,同时进行免疫粘附动力学观察。结果显示 ,鲫、草鱼两类细胞对内皮细胞的粘附率分别为 :0 .0 9± 0 .0 13,0 .2 0± 0 .0 18;0 .11± 0 .0 15,0 .2 1± 0 .0 2 3。表明鲤科鱼类不同种的同类细胞粘附率差异不大 ,而同种不同类细胞则差异显著。动力学观察分析表明 ,淋巴细胞粘附较快 ,60min进入平台期 ;单核细胞粘附慢 ,12 0min进入平台期。初步证明鱼类内皮细胞具有免疫介导作用。
Using the technique of endothelial cell (EC) culture in vitro in mammal, the artery endothelial cells were cultured and subcultured to the 3 rd generation and this 3rd generation artery EC was used as an adhesive material. The lymphocytes and mononuclear cells were isolated from the blood of grass carp and gold fish with the techniques of lymphocytes separation solution and glass adhesion. The endothelial cell adhesion was tested and a kinetic analysis was conducted. The results showed that the adhesive rate of lymphocytes and mononuclear cells in grass carp and gold fish were 0.11±0.015,0.21±0.023 and 0.09±0.013, 0.20±0.018, respectively. There were apparent differences between the 2 different cells in adhesive rates, but no evident differences between the 2 fishes. Adhesion of lymphocytes(60 min)was faster than that of mononuclear cells(120 min). Also, the immunity reaction of artery endothelial cells was primarily testified.
出处
《中国水产科学》
CAS
CSCD
北大核心
2001年第2期28-31,共4页
Journal of Fishery Sciences of China
关键词
鲤
内皮细胞
体外培养
鲫
草鱼
淋巴细胞
单细胞
粘附
免疫介导作用
common carp
endothelial cells
culture in vitro
gold fish
grass carp
lymphocytes, mononuclear cells
adhesion
