摘要
目的建立一种快速、简便、灵敏的分光光度法测定血清锌。方法在表面活性剂 Triton X- 10 0存在下 ,用 2 - (5 -溴 - 2 -吡啶偶氮 ) - 5 -二乙氨基酚 (5 - Br- PADAP)作显色剂 ,不去蛋白直接光度法测定血清锌。结果该方法显色络合物最大吸收波长为 5 5 8nm ,线性范围达 5 1.0μm ol/ L ,表观摩尔吸光数为 1.0 5× 10 5 L· mol- 1 · cm- 1 。回收率为 98.1%～ 10 3.2 % ,批内和批间变异系数 (CV)分别为 2 .1%与 3.6 % ,与原子吸收分光光度法比较相关良好 ,Y=1.0 2 X- 0 .41,r=0 .986 2 ,P>0 .0 5。 48名健康人血清锌含量为 (9.5～ 2 4.3)μmol/ L (x± 2 s)。结论该法血清用量少 ,不必去蛋白 ,具有操作快速、简便、结果灵敏可靠等优点 。
Objective To establish a simple. sensitive and stable method of determination of zinc in serum by UV spectrophotometry. Methods Determination of serum zinc with 2 (5 bromo 2 pyridylazo) 5 diethylaminophenol(5 Br PADAP) in the presence of surfactant Triton X 100. Results The maximum absorption of the complex compound was at 558 nm, the linearity of test was up to 51 μmol/L. The molar absorption coefficient is 1 05×10 5L/mol -1 ·cm -1 .The recoveries ranged from 98 1% to 103%. The within run and between run CV are 2 1% and 3 6%. Comparing this method (Y) with AAS(X), we obtained the regression equation.Y=1 02X-0 41,r=0 9862,P>0 05. The reference interval (±2s) was (9 5～24 3)μmol/L (n=48). Conclusions This method is simple, sensitive, stable, rapid and accurate for clinical application.
出处
《上海医学检验杂志》
北大核心
2001年第3期142-143,共2页
Shanghai Journal of Medical Laboratory Sciences
基金
浙江教委科研基金资助!(930 0 5 )
