摘要
目的 为探讨苦参碱抑制HepG2增殖的机制。方法 以不同浓度苦参碱作用于HepG2细胞不同时间用PI及AnnexinV FITC/PI双标记两法检测凋亡细胞。结果 0 8,1 5 ,2 0g/L苦参碱处理细胞 72h ,细胞均出现了凋亡峰 ,凋亡细胞分别为 10 5 7,16 37,10 85 % ;细胞早期凋亡检测 :2 0 ,1 5 ,3 0g/L苦参碱分别作用 6h、12h、12h ,凋亡细胞分别达 7 7,8 6 ,15 7% ;当 3 0g/L苦参碱作用 12h ,坏死细胞达 2 1 9%。结论 一定浓度苦参碱可诱导HepG2细胞凋亡 ,凋亡与坏死相伴存在并呈剂量和时间依赖性。
Objective To study the function of matrine in inhibiting proliferation of HepG2.Methods The cells were treated with 0～2 0 g/L matrine for 24～72 h,marked by PI,and with 0～3 0 g/L matrine for 24～72 h,marked by AnnexinV FITC /PI.Cell apoptosis was assayed by FCM.Results HepG2 apoptosis peak appeared after they were treated by 0 8,1 5,2 0 g/L matrine for 72 h,the percentage fo apoptosis cells were 10 57%,16 37%,and 10 85% respectively.Early stage apoptosis examination:when the cells were treated with 2 0,1 5,3 0 g/L matrine for 6,12,12 h,their percentages of apoptosis cells were 7 7,8 6,15 7% respectively.At the same time,nercrosis cells were increased,when the cells were treated with 3 0 g/L matrine for 12 h,necrosis cells rose to 21 9%.Conclusion Matrine in certain concentrations can induce HepG2 cells apoptosis and necrosis co exist, depending on matrine's affecting dosages and time.$$$$
出处
《肿瘤》
CAS
CSCD
北大核心
2001年第3期213-214,共2页
Tumor
