摘要
对孕兔子宫内膜的对比消化及统计分析表明 ,用浓度为 2 .5g/L或 1 .2 5g/L的胰酶 0～ 4℃消化7h3 0 min至 8h55min,可分离到正常的子宫内膜淋巴细胞 ( EML) ;培养条件单独作用或与酶浓度共同作用分别可极显著影响 ( P<0 .0 1 )或显著影响 ( P<0 .0 5)孕兔子宫内膜淋巴细胞的活化作用。试验表明 ,孕兔子宫内膜经 1 .2 5g/L的胰酶 0～ 4℃消化 8～ 9h,所获淋巴细胞在体外培养 4 8h是孕兔子宫内膜淋巴细胞制备及体外培养的可行方案 ;培养出的淋巴细胞适合用噻唑蓝 ( MTT)比色法进行活性检测。
Comparative digestion of endometrium lymphocytes (EML) from pregnant rabbits and variance analysis showed that intact EML could be isolated from endometria digested with trypsin(2.5 g/L or 1.25 g/L) for 7 h 30 min to 8 h 55 min(0～4℃) and the activation of the lymphocytes could be affected by culture condition( P <0.01) or by that and trypsin concentration together( P <0.05).It can be concluded that digestion of endometrium with 1.25 g/L trypsin for 8～9 h (0～4℃) was the optimal condition for preparation of EML from pregnant rabbit and the activation of EML obtained could be conveniently tested with MTT assay after 48 hours of culture in vitro.
出处
《西北农业学报》
CAS
CSCD
2001年第2期13-16,共4页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金!资助项目 (编号 :3 9970 5 44)
关键词
孕兔
子宫内膜淋巴细胞
消化
体外培养
Pregnant rabbit
Endometrium lymphocyte (EML)
Digestion
Culture in vitro
