摘要
采用 311- A最优回归设计 ,对落叶松胚性细胞诱导中激素种类与浓度优化筛选 ,建立胚性愈伤组织量依 2 ,4 - D、BA、KT的多项式回归方程。分析了试验因子的主效应和互作效应 ,借助此方程获得了 3因子的最佳配比组合以及最佳胚性愈伤组织发生量。 2 ,4 - D为 1.2 9mg· L-1、BA为0 .39mg· L-1和 KT为 0 .58mg· L-1时 ,落叶松胚性愈伤组织的诱导量可达到 13.9317mg·个 -1(外植体 )。结果表明 ,这是一种简便、实用。
The effects of 2,4-D, BA and KT on induction embryogenic callus (EC) of L. principis-rupprechtii were carried out by 311-A regression design. The regression equation was established which expresses the height of induction EC of L. principis-rupprechtii as function, and the 2,4-D, BA and KT as variable respectively. Using the regression equation, the single factor effect and mutual effect between the height of induction EC of L. principis-rupprechtii and 2,4-D, BA and KT was studied; the optimum concentration recipes can be obtained by computer processing, that are 2,4-D: 1.29 mg·L -1 ,BA: 0.39 mg·L -1 and KT: 0.58 mg·L -1 , the target height of induction EC is 13.931 7 mg per explant. The results of experiment showed that this method is simple, practical and rapid for selecting several hormone category and concentration recipes media of conifer somatic embryogenesis.
出处
《林业科学研究》
CSCD
北大核心
2001年第3期251-257,共7页
Forest Research
基金
国家自然科学基金项目! (39870 6 32
39770 6 2 9)
国家林业局"948"项目! (98- 4- 0 4- 0 2 )资助
关键词
落叶松
胚性愈伤组织
311-A最优回归设计
主效应互作效应
激素
培养基
Larix principis-rupprechtii
weight of induction embryogenic
311-A regression design
callus (EC)
single factor effect
mutual effect
