摘要
目的 :探讨nm2 3和c myc基因表达与睑板腺癌生物学行为的关系。方法 :采用原位杂交技术和免疫组化SABC法对 3 3例睑板腺癌石蜡切片中nm2 3和c myc基因的表达进行研究。 结果 :睑板腺癌nm2 3基因mRNA阳性表达率为 4 8.5 % ( 16/ 3 3 ) ,c myc基因阳性表达率为 5 4 .5 % ( 18/ 3 3 )。ⅡⅢ度睑板腺癌nm2 3基因mR NA表达阳性率为 63 .6% ( 14 / 2 2 ) ,Ⅰ度为 18.2 % ( 2 / 11) ,二者比较P <0 .0 5 ,中、低分化睑板腺癌nm2 3基因mR NA阳性表达率为 60 .9% ( 14 / 2 3 ) ,高分化癌nm2 3基因mRNA阳性率为 2 0 % ( 2 / 10 ) ,P <0 .0 5。ⅡⅢ度睑板腺癌c myc基因阳性表达率为 68.2 % ( 15 / 2 2 ) ,Ⅰ度为 2 7.3 % ( 3 / 11) ,P <0 .0 5 ;中低分化睑板腺癌c myc基因阳性表达率为 69.6% ( 16/ 2 3 ) ,高分化癌为 2 0 % ( 2 / 10 ) ,P <0 .0 5。nm2 3基因与c myc基因表达相关性检验 ,P >0 .0 5。结论 :nm2 3和c myc基因与睑板腺癌的发生、发展有关 ,与睑板腺癌分化程度密切相关 ,可能是判断其恶性程度的指标之一。
Objective: Our purpose was to investigate the relationship between expressions of nm23, c myc and biological progress of meibomian gland carcinoma. Methods: Expressions of nm23 and c myc were evaluated with in situ hybridization and immunohistochemical SABC method in 33 patients with meibomian gland carcinoma. Results: Positive rate of nm23 mRNA in meibomian gland carcinoma was 48.5% (16/33), and positive rate of c myc expression was 54.6% (18/33). Expression of nm23 was positive in 60.9% (14/23) of grade 2 to 3, 18.2% (2/11) in grade 1 (P< 0.05 ). Positive rate of nm23 in poorly differentiated group was 60.9% (14/23) and 20%(2/10) in well differentiated group (P<0.05). Positive expression of c myc in grade 1 to 2 was 68.2% (15/22), and 27.3% (3/11) in grade 1 (P<0.05). Positive rate of c myc in poorly differentiated group was 69.6% (16/23) and 20% (2/10) in well differentiated group(P<0.05). Conclusion: The gene of nm23 and c myc is related to the formation of meibomian gland carcinoma, which associated with the progress of its development and differentiation. They may act as malignant predictors in meibomian gland carcinoma.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2001年第4期311-313,共3页
Journal of China Medical University
