摘要
在比较芦丁、香蒲新苷和蒲黄总黄酮 (TYTF)经硝酸铝—亚硝酸钠—氢氧化钠试液及三氯化铝试剂显色前后的紫外可见吸收光谱的基础上 ,建立了TYTF的含量测定方法 :以香蒲新苷为对照品 ,蒲黄样品经聚酰胺柱分离净化 ,三氯化铝试剂显色后 ,采用紫外—可见分光光度法 ,在 4 0 5nm波长处测定TYTF含量。该方法较以芦丁为对照品 。
The method for the quantitative determination of total flavone in Pollen Typhae (TFPT) was developed on the basis of the comparison of ultraviolet visible absorption spectra of rutin, typhaneoside and TFPT before and after their coloration by the test solution of aluminium nitrate sodium nitrite sodium hydroxide and the reagent of aluminium trichloride. The sample was treated through a polyamide column for separation and purification, and was colorated with aluminium trichloride, and then, the content of TFPT in the sample was determined at the wavelength of 405 nm in an ultraviolet visible spectrophotometer with typhaneoside as a control. This method for the determination of TFPT was more rational than that in which the control was rutin, and the color reagent was aluminium nitrate sodium nitrite sodium hydroxide or aluminium trichloride.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2001年第4期23-25,共3页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金课题 (No .3 0 0 0 0 2 2 0 )
关键词
蒲黄总黄酮
香蒲新苷
紫外-可见分光光度法
芦丁
Total Flavone in Pollen Typhae
Typhaneoside
Ultraviolet visible Spectrophotometry
